Renilla Luciferase Vs Firefly Luciferase
(DLR™) gene assays This technical note describes the implementation of the DLR Assay System on Tecan’s next generation highend multimode reader platform Spark It can perform high sensitivity flash and glow luminescence measurements with a.
Renilla luciferase vs firefly luciferase. The cotransfection assays were performed in 12well plates with Lipofectamine 3000 reagent (Invitrogen, Grand Island, NY, USA) according to the manufacturer’s instructions and harvested after 24 h Finally, dualluciferase reporter assay system (Promega, Madison, WI, USA) was used to examine the activity of renilla and firefly luciferase. Reporter Assay System (Promega). (DLR™) gene assays This technical note describes the implementation of the DLR Assay System on Tecan’s next generation highend multimode reader platform Spark It can perform high sensitivity flash and glow luminescence measurements with a.
Jan 09, 21At 48 h after transfection, the luciferase assays were performed in manufacturer’s protocol using DualLuciferase Reporter Assay System (Promega) Renilla luciferase activity was measured using a Synergy HTX microplate reader (BioTek, Winooski, VT, USA), and the results were normalized using the activity of firefly luciferase. Cells were collected 36 h after transfection Additionally, firefly and renilla luciferase activity were detected by the Dual Luciferase Reporter Assay System.
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